The genome sequence of Azotobacter vinelandii , an obligate 1 aerobe specialized to support diverse anaerobic metabolic 2 processes 3 4 running title : Genome sequence of Azotobacter vinelandii

نویسندگان

  • João C. Setubal
  • Patricia dos Santos
  • Barry S. Goldman
  • Helga Ertesvåg
  • Guadelupe Espin
  • Luis M. Rubio
  • Svein Valla
  • Nalvo F. Almeida
  • Kaitlyn Hellner-Burris
  • José A. Hernandez
  • Katherine Houmiel
  • Christina Kennedy
  • Timothy J. Larson
  • Phil Latreille
  • Lauren S. Ligon
  • Jing Lu
  • Mali Mærk
  • Nancy M. Miller
  • Stacie Norton
  • Ina P. O’Carroll
  • Estella C. Raulfs
  • Rebecca Roemer
  • James Rosser
  • Daniel Segura
  • Steve Slater
  • Shawn L. Stricklin
  • David J. Studholme
  • Jian Sun
  • Carlos J. Viana
  • Erik Wallin
  • Baomin Wang
  • Cathy Wheeler
  • Huijun Zhu
  • Dennis R. Dean
چکیده

6 João C. Setubal, Patricia dos Santos, Barry S. Goldman, Helga Ertesvåg, 7 Guadelupe Espin, Luis M. Rubio, Svein Valla, Nalvo F. Almeida, Divya 8 Balasubramanian, Lindsey Cromes, Leonardo Curatti, Zijin Du, Eric Godsy, Brad 9 Goodner, Kaitlyn Hellner-Burris, José A. Hernandez, Katherine Houmiel, Juan 10 Imperial, Christina Kennedy, Timothy J. Larson, Phil Latreille, Lauren S. Ligon, 11 Jing Lu, Mali Mærk, Nancy M. Miller, Stacie Norton, Ina P. O’Carroll, Ian 12 Paulsen, Estella C. Raulfs, Rebecca Roemer, James Rosser, Daniel Segura, 13 Steve Slater, Shawn L. Stricklin, David J. Studholme, Jian Sun, Carlos J. Viana, 14 Erik Wallin, Baomin Wang, Cathy Wheeler, Huijun Zhu, Dennis R. Dean, Ray 15 Dixon, Derek Wood 16

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Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes.

Azotobacter vinelandii is a soil bacterium related to the Pseudomonas genus that fixes nitrogen under aerobic conditions while simultaneously protecting nitrogenase from oxygen damage. In response to carbon availability, this organism undergoes a simple differentiation process to form cysts that are resistant to drought and other physical and chemical agents. Here we report the complete genome ...

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The cydR gene product, required for regulation of cytochrome bd expression in the obligate aerobe Azotobacter vinelandii, is an Fnr-like protein.

The cytochrome bd complex in the obligately aerobic diazotroph Azotobacter vinelandii is an oxidase, which, in vivo, has a low affinity for oxygen and is required for respiratory protection of nitrogenase. Mutations caused by insertion of Tn5-B20 upstream of the structural genes (cydAB) for cytochrome bd result in over-expression of this oxidase and, for unexplained reasons, inability of the or...

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Complete Genome Sequences of Azotobacter vinelandii Wild-Type Strain CA and Tungsten-Tolerant Mutant Strain CA6

We report the complete genome sequences of Azotobacter vinelandii mutant strain CA6 and its parent wild-type strain, CA. When fixing nitrogen, strain CA6 displays slow growth and impaired molybdate uptake, tolerance to tungstates, and production of hydrogen gas, compared to results for strain CA. Comparing these genome sequences may provide a genetic basis for these mutant phenotypes.

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Characterization of Azotobacter vinelandii deoxyribonucleic acid and folded chromosomes.

The properties of Azotobacter vinelandii deoxyribonucleic acid (DNA) and folded chromosomes were studied and compared to those of Escherichia coli as a standard. Based on melting temperature and buoyant density measurements, the guanosine + cytosine content of purified A. vinelandii DNA was 65%, whereas that of E. coli DNA was 50%. The results of renaturation studies showed that the unique DNA ...

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Azotobacter vinelandii mutS: nucleotide sequence and mutant analysis.

An Azotobacter vinelandii homolog to the Salmonella typhimurium mutS gene was discovered upstream of the fdxA gene. The product of this gene is much more similar to S. typhimurium MutS than either is to the HexA protein of Streptococcus pneumoniae. An A. vinelandii delta mutS mutant strain was shown to have a spontaneous mutation frequency 65-fold greater than that of the wild type.

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تاریخ انتشار 2009